Journal: BMC neuroscience

Article Title: Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α 2A -adrenergic receptor.

doi: 10.1186/s12868-024-00845-4

Figure Lengend Snippet: Fig. 4 Expression changes of ARs in rats exposed to noise and controls. a A schematic diagram illustrating the experimental procedure. b Western blot showing the expression changes of ARs in rats exposed to noise and controls (n = 6). c Quantification of the western blotting results. *p < 0.05, **p < 0.01. d Representative images showing DBH immunostaining at different positions along the base-to-apex axis. Rows and columns correspond to different positions along the cochlear axis, respectively, as indicated. Scale bar: 50 μm. e-f Representative images showing α2A-AR immunostaining at Organ of Corti and SGNs. Scale bar: 50 μm

Article Snippet: The blots were cut prior to hybridisation with antibodies during blotting, the membranes were incubated with specific primary antibodies: anti-α1A-specific polyclonal antibody (1:1000, 19777-1-AP, Proteintech), anti-α1B antibody (1:1000, DF8798, Affinity), anti-α1D antibody (0.2 μg/ml, ab166925, Abcam), anti-α2A polyclonal antibody (1:1000, 14266-1-AP, Proteintech), anti-α2B antibody (1:1000, A8535, ABclonal), anti-α2C antibody (1:1000, DF3108, Affinity), anti-β1 antibody (1:1000, bs-0498R, Bioss), anti-β2 antibody (1:1000, DF3512, Affinity), anti-β3 antibody (1:1000, bs-1063R, Bioss) and anti-GAPDH polyclonal antibody (1:10000, 10494-1-AP, Proteintech) overnight at 4 °C.

Techniques: Expressing, Western Blot, Immunostaining

Journal: BMC neuroscience

Article Title: Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α 2A -adrenergic receptor.

doi: 10.1186/s12868-024-00845-4

Figure Lengend Snippet: Fig. 5 Schematic diagram of potential protective mechanisms of NE against NIHL via α2A-AR. The levels of ROS increased after noise exposure in OHCs, leading to cell death and NIHL. Treatment with NE, an activator of α2A-AR, attenuated oxidative stress and protected from cell injury

Article Snippet: The blots were cut prior to hybridisation with antibodies during blotting, the membranes were incubated with specific primary antibodies: anti-α1A-specific polyclonal antibody (1:1000, 19777-1-AP, Proteintech), anti-α1B antibody (1:1000, DF8798, Affinity), anti-α1D antibody (0.2 μg/ml, ab166925, Abcam), anti-α2A polyclonal antibody (1:1000, 14266-1-AP, Proteintech), anti-α2B antibody (1:1000, A8535, ABclonal), anti-α2C antibody (1:1000, DF3108, Affinity), anti-β1 antibody (1:1000, bs-0498R, Bioss), anti-β2 antibody (1:1000, DF3512, Affinity), anti-β3 antibody (1:1000, bs-1063R, Bioss) and anti-GAPDH polyclonal antibody (1:10000, 10494-1-AP, Proteintech) overnight at 4 °C.

Techniques: